Podol'sk, Moscow, Russian Federation
The purpose of the study is improvement of pedigree accessory of identification process of poultry with use of microsatellite markers. The analysis was performed with 9 breeds of chickens (n=268) for 16 markers. The studies were performed with the chicken breeds, such as: the Black and White Аustralorp (BWAus), Black Australorp (BAus), Kulangi (Kul), Czech Gold (CzG), Blue Andalusian (BAnd), Naked Neck (NN), Silver Campine (Breukel) (SC), Black Bearded Russian (BBRus), and Minorca (Min). There were 29 to 30 poultry in each experimental group. The researches were carried out at the Molecular Genetics Laboratory, Ernst All-Russia Research Institute of Animal Husbandry. Separation and detection of amplification products were performed on an Applied Biosystems АВI3130xl Genetic Analyzer (USA). Statistical data processing was performed using GenAlEx software version 6.4. The work examined genetic diversity of chickens (average number of alleles, number of effective alleles, the level of homo - and heterozygosity, etc.), using microsatellite loci. It should be noted that the average number of alleles in the study ranged from 6.06±0.050 in the BAnd bantams to 7.81±0.73 in the NN chickens, while the number of informative alleles ranged from 3.63±0.30 in the BAnd bantams to 5.44±0.34 in the SC chickens. High informativeness of 16 selected loci performance was noted during the research; the number of alleles ranged from 8 (locus MCW0037) to 25 alleles (locus MCW0183), depending on the locus. The conducted research allowed us to identify the presence of private alleles in the studied breeds of chickens, which were discovered in poultry within 7 of 9 breeds with frequencies of 1.7 to 67.7%. The expected and observed levels of heterozygosity were calculated to assess the variation of the investigated species more accurately; their mean values reached 66.9 and 37.1%, respectively. Breeds of chickens affiliated to their populations were identified based on analysis of 16 microsatellite markers; the estimates varied from 79.3% to 100.0%. Therefore, the use of microsatellite loci in chicken breeding can give more opportunities to create pedigree poultry flocks within the genetic pool of breeds.
microsatellite, markers, alleles, breed, chicken, genetic, diversity
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