The aim of the study was to detect significant biochemical changes in antioxidant status during acute period of ischemic stroke in its various pathogenic subtypes in patients aged 45–74years. The most important results were blood levels of ascorbic acid, malondialdehyde and free/ oxidized glutathione ratio. Cardioembolic stroke was associated with: the reduced ascorbic acid level of 62–74% comparable to control group; 5-times increased malondialdehyde level at the end of the first week of the disease; and also 50% decreasing of cell antioxidant capacity comparable to control group and other stroke subtypes. In lacunary stroke minimal changes in antioxidant status were measured, possibly because of small size of brain damage. Positive clinical dynamics was followed by decreasing of free glutathione levels. It could be related with its previous significant expenditure in process of cell recovery in penumbra area. In case of favorable outcome all of the markers return to the levels which were comparable to control group. The differences in the dynam-ics of the cells antioxidant capacity and consumption of native antioxidants, the time from the onset of the disease, the severity of the clinical picture, depending on the stroke subtype, were revealed. Further research of this problem may help to create new methods of diagnostics and selective pharmacological correction of this pathology.
ischemic stroke, oxidative stress, antioxidants, glutathione, ascorbic acid, malondialdehyde, high performance liquid chromatography
1. .BarakhovskayaTV, KolesnichenkoLS, VerlanNV(2010). Free-radical damage in chronic cerebral ischemia and hypothyroid [Svobodnoradikal’noe povrezhdenie pri khronicheskoy tserebral’noy ishemii i gipotireoze]. Sibirskiy meditsinskiy zhurnal, (1), 39-40
2. VerlanNV, BardymovVV, KulinskiyVI, Shpra-khVV (2006). Activity of glutathione metabolism enzymes in patients with chronic cerebral ischemia[Aktivnost’ fermentov metabolizma glutationa u bol’nykh khronicheskoy ishemiey golovnogo mozga]. Chelovek i lekarstvo: Materialy XIII Rossiyskogo natsional’nogo kongressa, 86.
3. GusevEI, SkvortsovaVI, StakhovskayaLV (2007). Stroke problem in Russian Federation: time of active joint actions [Problema insul’ta v Rossiyskoy Federatsii: vremya aktivnykh sovmestnykh deystviy]. Zhurnal nevrologii i psikhiatrii im. S.S. Korsakova, 107(8), 4-10.
4. DutovAA, NikitinDA, TereshkovPP, MartynovaAV, SverkunovaAV, ErmolinaAV, LukyanovaYL, Shemyaki-naNA (2015). Simple HPLC-analysis of glutathione in erythrocytes [Prostoy VEZhKh-analiz glutationa v eritrotsitakh]. Voprosi biologitseskoy, meditsinskoy i farmatsevtitseskoy khimii, (6), 12-15.
5. DutovAA, NikitinDA, ShemyakinaNA, LukyanovaYL,ErmolinaAV (2015). Simple HPLC method of ascorbic acid detection in blood plasma [Prostoy VEZhKh metod opredeleniya askorbinovoy kisloty v plazme krovi]. European Journal of Analytical and Applied Chemistry, (2), 6-11.
6. MartynovMY, YasmanovaAN, KolesnikovaTI (2010). Oxidative stress in patients with stroke [Okislitel’nyy stress u bol’nykh s mozgovym insul’tom]. Consilium medicum. Nevrologiya i revmatologiya, (2), 14-17.
7. MuravyovaVN, KarpovaEN (2014). Modern con-ception of risk factors and prevention of stroke (re-view) [Sovremennye predstavleniya o faktorakh riska i profilaktiki ONMK (obzor literatury)]. Mezhdunarodnyy zhurnal eksperimental’nogo obrazovaniya, (2-3), 59-64.
8. ParfyonovVA, KhasanovaDR (2012). Ischemic stroke [Ishemicheskiy insul’t], 312.
9. PizovaNV, ChizhovPA, TabakovVA, Prozorovs-kayaSD, DmitrievAN (2011). Clinical features of acute cerebral blood flow disorders in young men with undifferentiated dysplasia syndrome of connecting tissue [Klinicheskie osobennosti ostrykh narusheniy mozgovogo krovoobrashcheniya u lits molodogo vozrasta s sindromom nedifferentsirovannoy displazii soedinitel’noy tkani]. Zhurnal nevrologii i psikhiatrii im. S.S. Korsakova, (2), 19-24.
10. SkvortsovaVI (2007). Reductuion of the morbidity, mortality and invalidization from stroke in Russian Federation [Snizhenie zabolevaemosti, smertnosti i invalidnosti ot insul’tov v Rossiyskoy Federatsii], 192.
11. SkvortsovaVI, NartsissovYR, BodykhovMK, Ki-chukIV, PryanikovaNA, GudkovaYV, SoldatenkovaTD,KondrashovaTT, KalininaEV, NovitchkovaMD, Shutye-vaAB, KerbikovOB (2007). Oxidative stress and oxygen status during ischemic stroke [Oksidantnyy stress i kislorodnyy status pri ishemicheskom insul’te]. Zhurnal nevrologii i psikhiatrii im. S.S.Korsakova, (1), 30-36.
12. StakhovskayaLV, KlochikhinaOA, BogatyrevaMD (2013). Stroke epidemiology in Russia in territorial population register results [Epidemiologiya insul’ta v Rossii po rezul’tatam territorial’no-populyatsionnogo registra (2009–2010)]. Zhurnal nevrologii i psikhiatrii im. S.S. Korsakova. Insul’t, (5), 4-10
13. DringenR (2000). Metabolism and functions of glutathione in brain. Progress in neurobiology, (62), 649-671.
14. MeisterA, AndersonME (1983). Glutathione. Ann. Rev. Biochem., (52), 711-760.
15. European Stroke Organisation (ESO) Executive Committee (2008). Guidelines for management of ischaemic stroke and transient ischaemic attack. Cerebrovascular Disease, 25(5), 457-507.
16. ZimmermannC, WinnefeldK, StreckS, RoskosM, HaberlRL (2004). Antioxidant status in acute stroke patients and patients at stroke risk. Eur. Neurol., 51(3), 157-161.