PECULIARITIES OF EFFECTIVE INDICATORS OF PHAGOCYTES AND MICROBIOLOGICAL PARAMETERS OF THE LOCAL FOCUS OF PERIAPICAL INFLAMMATION IN DIFFERENT FORMS OF CHRONIC PERIODONTITIS

Larisa Latyushina; Il&#039;ya Dolgushin; Anastasiia Lapteva; Ekaterina Kolbina; Aleksey Finadeev; Yuliya Pavlienko

doi:doi:10.18481/2077-7566-20-17-2-96-102

UDC 61

PECULIARITIES OF EFFECTIVE INDICATORS OF PHAGOCYTES AND MICROBIOLOGICAL PARAMETERS OF THE LOCAL FOCUS OF PERIAPICAL INFLAMMATION IN DIFFERENT FORMS OF CHRONIC PERIODONTITIS

Published в Actual problems in dentistry · Volume 17, Issue 2, 2021 · Pages 96–102 · Rubrics: SURGICAL DENTISTRY AND IMPLANTOLOGY

DOI 10.18481/2077-7566-20-17-2-96-102

Received: 09.08.2021 Accepted: 12.08.2021 Published: 12.08.2021 Language of publication: RUS

Authors

Larisa Latyushina ¹ · Il'ya Dolgushin ² · Anastasiia Lapteva ³ · Ekaterina Kolbina ⁴ · Aleksey Finadeev ⁵ · Yuliya Pavlienko ⁶

1 Ural State Medical University (Department of Dental and Maxillofacial surgery Department, Professor of the Department)
Chelyabinsk, Chelyabinsk, Russian Federation

2 Yuzhno-Ural'skiy gosudarstvennyy medicinskiy universitet
Chelyabinsk, Russian Federation

3 South Ural State Medical University (Department of Surgical Dentistry and Maxillofacial Surgery, assistant of the department)
from 01.01.2017 to 01.01.2020 Chelyabinsk, Chelyabinsk, Russian Federation

4 Yuzhno-Ural'skiy gosudarstvennyy medicinskiy universitet (Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics, Docent of Department)
Chelyabinsk, Chelyabinsk, Russian Federation

5 South Ural State Medical University
Chelyabinsk, Chelyabinsk, Russian Federation

6 South Ural State Medical University
Chelyabinsk, Chelyabinsk, Russian Federation

ABSTRACT

In the last decade, single-stage dental implantation has become widespread, often with immediate loading. A significant part of the implants are installed in the zone of chronic periapical inflammation, however, until now there are no data on the relationship between the form of chronic periodontitis and the processes of osseointegration in the peri-implant zone. The aim of this study was to study the effector parameters of wound neutrophils and the spectrum of pathogens of the local focus of inflammation in various forms of chronic peridontitis. 74 surveyed persons were divided into four groups, depending on the form of chronic periodontitis: group 1 was represented by patients with chronic fibrous periodontitis; Group 2 — patients with chronic granulating periodontitis; Group 3 — subjects with chronic granulomatous periodontitis; Group 4 — control group, patients with healthy teeth removed for orthopedic or orthodontic indications. We studied the quantitative composition of wound phagocytes and functional parameters of wound neutrophils of the separated socket of the extracted tooth, and also studied the biocenosis. Comparative analysis of the effector indices of neutrophils of the socket of the extracted tooth of the patients of the control group and those examined with various forms of chronic periapical inflammation made it possible to reveal in the latter: a twofold increase in the relative activity of phagocytosis and phagocytic number; multiple excess of the control value of the indicators of the intensity of the phagocytic reaction; high rates of activity and intensity of spontaneous and induced NBT-reducing function of wound phagocytes. Microbiological research made it possible to determine the differences in the composition of the microflora of the detachable sockets of the extracted teeth in patients with various forms of chronic periodontitis. Despite statistically significant differences in a number of immunological parameters and the revealed different microbial spectrum of pathogens of a chronic periapical focus, in patients with all forms of chronic periodontitis, predictors of the development of inflammatory complications were identified in the context of the planned one-stage dental implantation when implants were installed in the sockets of teeth with chronic periodontal foci.

KEYWORDS

one-stage (immediate) intraosseous dental implantation chronic periodontitis functional activity of neutrophils microbiological examination periapical inflammation

Information References

Authors:

1. Ural State Medical University (Department of Dental and Maxillofacial surgery Department, Professor of the Department)

Chelyabinsk, Chelyabinsk, Russian Federation

2. Yuzhno-Ural'skiy gosudarstvennyy medicinskiy universitet

Chelyabinsk, Russian Federation

3. South Ural State Medical University (Department of Surgical Dentistry and Maxillofacial Surgery, assistant of the department)
from 01.01.2017 to 01.01.2020
Chelyabinsk, Chelyabinsk, Russian Federation

4. Yuzhno-Ural'skiy gosudarstvennyy medicinskiy universitet (Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics, Docent of Department)

Chelyabinsk, Chelyabinsk, Russian Federation

5. South Ural State Medical University

Chelyabinsk, Chelyabinsk, Russian Federation

6. South Ural State Medical University

Chelyabinsk, Chelyabinsk, Russian Federation

Type:

Article

Pages:

from 96 to 102

Status:

Published

Subject area:

UDC 61

Language:

Russian

Keywords:

one-stage (immediate) intraosseous dental implantation, chronic periodontitis, functional activity of neutrophils, microbiological examination, periapical inflammation

References

1. Pohoden'ko-Chudakova I.O., Luckaya I.K., Shevela T.L. i dr. Vliyanie etiologicheskih faktorov, privodyaschih k chastichnoy vtorichnoy adentii, na chastotu razvitiya posleoperacionnyh vospalitel'nyh oslozhneniy pri neposredstvennoy dental'noy implantacii. BGMU: 90 let v avangarde medicinskoy nauki i praktiki : sbornik nauchnyh trudov. Minsk : Belorusskiy gosudarstvennyy medicinskiy universitet. 2014:239-241. [I.O. Pokhodenko-Chudakova, I.K. Lutskaya, T.L. Shevela et al. Influence of etiological factors leading to partial secondary adentia on the incidence of postoperative inflammatory complications during direct dental implantation. BSMU: 90 years at the forefront of medical science and practice : collection of scientific papers. Minsk : Belarusian State Medical University. 2014:239-241. (In Russ.)]. https://elibrary.ru/item.asp?id=32878738

2. Dolgushin I.I., Buharin O.V. Neytrofily i gomeostaz. Ekaterinburg. 2001:259. [I.I. Dolgushin, O.V. Bukharin. Neutrophils and homeostasis. Yekaterinburg. 2001:259. (In Russ.)]. https://elibrary.ru/item.asp?id=18447336

3. Dolgushin I.I. Rol' neytrofilov v regulyacii antimikrobnoy rezistentnosti. Vestnik Rossiyskoy akademii medicinskih nauk. 2002;3:16-20. [I.I. Dolgushin. The role of neutrophils in the regulation of antimicrobial resistance. Bulletin of the Russian Academy of Medical Sciences. 2002;3:16-20. (In Russ.)], https://elibrary.ru/item.asp?id=23723865

4. Durnovo E.A. Sravnitel'nyy analiz funkcional'noy aktivnosti neytrofilov krovi i rotovoy polosti u bol'nyh s gnoyno-vospalitel'nym processom v polosti rta. Stomatologiya. 2005;3:29-32. [E.A. Durnovo. Comparative analysis of the functional activity of blood and oral cavity neutrophils in patients with a purulent-inflammatory process in the oral cavity. Dentistry. 2005;3:29-32. (In Russ.)]. https://elibrary.ru/item.asp?id=41394982

5. Red. Smirnov V.S., Freydlin I.S. Immunodeficitnye sostoyaniya. SPb. 2000:561. [Eds. V.S. Smirnov, I.S. Freidlin. Immunodeficiency states. SPb. 2000:561. (In Russ.)].

6. Red. Frimel' G.M. Immunologicheskie metody. Moskva : Medicina. 1987:472. [Ed. G.M. Frimel. Immunological methods. Moscow : Medicine. 1987:472. (In Russ.)].

7. Red. Oleynikov E.A., Ebert L.Ya. Immunologicheskie metody issledovaniya. Uchebnoe posobie. Saransk. 1981:92. [Eds. E.A. Oleinikov, L.Ya. Ebert. Immunological research methods. Textbook. Manual. 1981:92. (In Russ.)].

8. Bolezni periapikal'nyh tkaney. Klinicheskie rekomendacii (protokoly lecheniya). Moskva. 2014:2018. [Reriapical tissue disease. Clinical guidelines (treatment protocols). Moscow. 2014:2018. (In Russ.)].

9. Latyushina L.S., Berezhnaya E.S., Dolgushin I.I., Finadeev A.P., Pavlienko Yu.V. Vliyanie immunoterapii rekombinantnym IL-1β na kliniko-immunologicheskie pokazateli pacientov s oslozhnennymi perelomami nizhney chelyusti. Problemy stomatologii. 2017;13(2):49-53. [L.S. Latyushina, E.S. Berezhnaya, I.I. Dolgushin, A.P. Finadeev, Yu.V. Pavlienko. The effect of immunotherapy with recombinant IL-1β on the clinical and immunological parameters of patients with complicated fractures of the mandible. Actual problems in dentistry. 2017;13(2):49-53. (In Russ.)]. https://elibrary.ru/item.asp?id=29767440

10. Latyushina L.S., Lapteva A.V., Plehanova E.V., Malysheva L.Yu., Shirshova N.E. Osobennosti funkcional'noy aktivnosti neytrofilov i urovnya citokinov pacientov s hronicheskim periodontitom. Problemy stomatologii. 2020;16(2):7-15. [L.S. Latyushina, A.V. Lapteva, E.V. Plekhanova, L.Yu. Malysheva, N.E. Shirshova. Features of the functional activity of neutrophils and the level of cytokines in patients with chronic periodontitis. Actual problems in dentistry. 2020;16(2):7-15. (In Russ.)]. https://elibrary.ru/item.asp?id=4378371

11. Latyushina L.S., Piotrovich A.V., Dolgushin I.I., Finadeev A.P., Pavlienko Yu.V. Vliyanie topicheskoy citokinoterapii rekombinantnym IL-2 (ronkoleykinom) na kliniko-immunologicheskie parametry pacientov pri provedenii zakrytogo sinus-liftinga s odnomomentnoy dental'noy implantaciey. Problemy stomatologii. 2018;14(1):83-88. [L.S. Latyushina, A.V. Piotrovich, I.I. Dolgushin, A.P. Finadeev, Yu.V. Pavlienko. Influence of topical cytokine therapy with recombinant IL-2 (roncoleukin) on the clinical and immunological parameters of patients during closed sinus lift with simultaneous dental implantation. Actual problems in dentistry. 2018;14(1):83-88. (In Russ.)]. https://elibrary.ru/item.asp?id=32840696

12. Lebedev K.A., Ponyakina I.D. Immunogramma v klinicheskoy praktike. Moskva : Nauka. 1990:224. [K.A. Lebedev, I.D. Ponyakina. Immunogram in clinical practice. Moscow : Nauka. 1990:224. (In Russ.)].

13. Lukinyh L.M., Livshic Yu.N. Verhushechnyy periodontit. Uchebnoe posobie. N. Novgorod : Izd-vo NGMA. 2004:92. [L.M. Lukinykh, Yu.N. Livshits. Apical periodontitis. Textbook. 2nd edition. Nizhny Novgorod : Publishing house of the Nizhny Novgorod State Medical Academy. 2004:92. (In Russ.)].

14. Mahrova T.V. Faktory i usloviya, vliyayuschie na fagocitarnuyu adgezivnuyu aktivnost' Candida albicans v sistemah s bukal'nymi epiteliocitami : dis. ... kand. med. nauk. N. Novgorod, 2004:132. [T.V. Makhrova. Factors and conditions affecting the phagocytic adhesive activity of Candida albicans in systems with buccal epithelial cells : dis. ... cand. med. sciences. N. Novgorod, 2004:132. (In Russ.)]. https://www.elibrary.ru/item.asp?id=15791298

15. Mayanskiy A.N. Mikrobiologiya dlya vrachey. N. Novgorod : Izd-vo NGMA. 1999:393. [A.N. Mayansky. Microbiology for doctors. N. Novgorod : Publishing house of NGMA. 1999:393. (In Russ.)].

16. Mayanskiy A.N., Viksman M.K. Sposob ocenki funkcional'noy aktivnosti neytrofilov cheloveka po reakcii vosstanovleniya nitrosinego tetrozoliya. Metod. rekomendacii. Kazan'. 1979:11. [A.N. Mayansky, M.K. Viksman. Method for assessing the functional activity of human neutrophils by the reduction reaction of nitro blue tetrozolium. Method. Recommendations. Kazan. 1979:11. (In Russ.)].

17. Mayanskiy A.N., Mayanskiy N.A., Zaslavskaya M.A. i dr. Apoptoz neytrofilov. Immunologiya. 1999;20.6:12-19. [A.N. Mayansky, N.A. Mayansky, M.A. Zaslavskaya et al. Apoptosis of neutrophils. Immunology. 1999;20.6:12-19. (In Russ.)].

18. Mitronin A.V. Kompleksnoe lechenie i reabilitaciya bol'nyh s destruktivnymi formami hronicheskogo periodontita : avtoref. dis. … doktor. med. nauk. 14.01.14. Moskva, 2004:50. [A.V. Mitronin. Complex treatment and rehabilitation of patients with destructive forms of chronic periodontitis. 14.00.21 : avtoref. dis. ... dkt. med. nauk. Moscow, 2004:50. (In Russ.)]. https://elibrary.ru/item.asp?id=15809674

19. Nikol'skiy V.Yu. Rannyaya i otsrochennaya dental'naya implantaciya (kliniko-eksperimental'noe issledovanie) : avtoref. dis. … doktor. med. nauk. 14.01.14. Samara, 2007:44. [V.Yu. Nikolsky. Early and delayed dental implantation (clinical and experimental study). 14.00.21 : avtoref. dis. ... dkt. med. nauk. Samara, 2007:44. (In Russ.)]. https://elibrary.ru/item.asp?id=30306309

20. Raad Z. Rezul'taty neposredstvennoy dental'noy implantacii posle udaleniya zubov po povodu hronicheskogo periodontita i tyazheloy formy generalizovannogo parodontita. Estestvennye i tehnicheskie nauki. 2009;2.40:164-169. [Z. Raad. Results of direct dental implantation after tooth extraction for chronic periodontitis and severe generalized periodontitis. Natural and technical sciences. 2009;2.40:164-169. (In Russ.)]. https://elibrary.ru/item.asp?id=14308577

21. Predtechenskiy V.E. Rukovodstvo po klinicheskim laboratornym issledovaniyam. Moskva : Medicina. 1964:960. [V.E. Predtechensky. Guidelines for clinical laboratory research. Moscow : Medicine. 1964:960. (In Russ.)].

22. Sablina G.I., Kovtonyuk P.A., Soboleva N.N. i dr. Sistematika hronicheskih periodontitov i ih mesto v MKB-10. Sibirskiy medicinskiy zhurnal. 2011;105.6:300-302. [G.O. Sablina, P.A. Kovtonyuk, N.N. Soboleva et al. Systematics of chronic periodontitis and their place in ICD-10. Siberian medical journal. 2011;105.6:300-302. (In Russ.)]. https://elibrary.ru/item.asp?id=17053603

23. Simbircev A.S. Citokiny - novaya sistema regulyacii zaschitnyh reakciy organizma. Citokiny i vospalenie. 2002;1(1):9-16. [A.S. Simbirtsev. Cytokines are a new system for regulating the body's defense reactions. Cytokines and inflammation. 2002;1(1):9-16. (In Russ.)].

24. Sirak S.V., Kazieva I.E. Sovershenstvovanie metodov neposredstvennoy i ranney otsrochennoy dental'noy implantacii u pacientov s defektami zubnyh ryadov. Sovremennye problemy nauki i obrazovaniya. 2013;4:100. [S.V. Sirak, I.E. Kazieva. Improving the methods of immediate and early delayed dental implantation in patients with dentition defects. Modern problems of science and education. 2013;4:100. (In Russ.)]. https://elibrary.ru/item.asp?id=20848179

25. Suleymanova A.R., Vorotnyak Yu.Yu., Kostina I.N. Kliniko-morfologicheskaya diagnostika periapikal'noy patologii zuba. Aktual'nye voprosy sovremennoy medicinskoy nauki i zdravoohraneniya : materialy III Mezhdunarodnoy nauchno-prakticheskoy konferencii molodyh uchenyh i studentov, III Foruma medicinskih i farmacevticheskih VUZov Rossii "Za kachestvennoe obrazovanie". Ekaterinburg : UGMU. 2018:325-331. [A.R. Suleimanova, Yu.Yu. Vorotnyak, I.N. Kostina. Clinical and morphological diagnostics of periapical tooth pathology. Actual issues of modern medical science and health care : Proceedings of the III International scientific and practical conference young scientists and students, III Forum of Medical and Pharmaceutical Universities of Russia "For Quality Education". Yekaterinburg : Ural State Medical University. 2018:325-331. (In Russ.)]. https://elibrary.ru/item.asp?id=37271029

26. Totolyan A.A., Freydlin I.S. Kletki immunnoy sistemy. Spb. : Nauka. 2000:231. [A.A. Totolyan, I.S. Freidlin. Cells of the immune system. Spb. : Science. 2000:231. (In Russ.)]. https://www.elibrary.ru/item.asp?id=20532673

27. Robustova T.G. Hirurgicheskaya stomatologiya. Uchebnik. 3-e izd., pererab. i dop. Moskva : Medicina. 2003:504. [T.G. Robustova. Surgical dentistry. Textbook. 3rd ed., Rev. and add. Moscow : Medicine. 2003:504. (In Russ.)].

28. Carev V.N., Ushakov R.V. Antimikrobnaya terapiya v stomatologii. Dlya slushateley sistemy poslevuz. podgotovki vrachey-stomatologov. Moskva : MIA. 2004:143. [V.N. Tsarev, R.V. Ushakov. Antimicrobial therapy in dentistry. For students of the postgraduate system. training of dentists. Moscow : MIA. 2004:143. (In Russ.).

29. Adell R., Lekholm U., Rockler B., R., Branemark P.-I. A 15-year study of osseointegrated implants in the treatment of the edentuious jaw // Int. J. Oral Surg. - 1981;10:387-416.

30. Branemark P.L. Intraduction to osseointegration. Tissue - Integrated Prostheses: Osseointegration in Clinical Dentistry // Chicago: Quintessence. - 1985:11-76.

31. Brinkmann V., Rechard U., Goosmann C. et al. Neutrophil extracelllulartraps kill bacteria // Sciense. - 2004;303:1532-1535.

This work is licensed under Creative Commons Attribution 4.0 International

PECULIARITIES OF EFFECTIVE INDICATORS OF PHAGOCYTES AND MICROBIOLOGICAL PARAMETERS OF THE LOCAL FOCUS OF PERIAPICAL INFLAMMATION IN DIFFERENT FORMS OF CHRONIC PERIODONTITIS

Confirmation

Регистрация