employee
, Russian Federation
employee
, Russian Federation
GRNTI 68.41 Ветеринария
The article presents the results of the clinical application of new technology — UV-corneal collagen cross-linking procedure, which we use in the complex therapy of severe corneal pathologies for dogs and cats: septic ulcers complicated by keratomalacia, keratectasias of various origins, endothelial-epithelial corneal dystrophies. The UV cross-linking method is often performed by us as a stage in the preparation of a donor graft during keratoplasty operations in dogs and cats. The effect of the cross-linking treatment procedure is achieved by photopolymerizing collagen fibers of corneal stroma under the combined action of photosensitizer (riboflavin solution) and ultraviolet radiation with a certain wavelength. After cross-linking, it is possible to achieve increase in the biomechanical and biochemical stability of the cornea, to observe dehydration and antimicrobial effects. The article contains information about the stages and results of several methods of UV-corneal collagen cross-linking, which we successfully use in clinical practice in dogs and cats: A-CXL method, PACK-CXL, M-CXL, method of cross-linking modification of donor heterologous graft as a stage in performing keratoplasty surgery to recipient. Based on our clinical results, we can conclude that corneal cross-linking in dogs and cats has a high therapeutic potential and is a promising method for treating severe keratopathies in cases of weak effectiveness of traditional drug therapy regimens.
corneal collagen cross-linking, corneal ulcers, corneal melting, keratectasis, endothelial-epithelial corneal dystrophy, riboflavin, corneal transplantation, dogs, cats
1. Borhunova, E.N. Deystvie sekretoma stvolovyh kletok (citokinov) na vospalitel'nye i reparativnye processy pri inducirovannoy yazve rogovicy / E.N. Borhunova, S.V. Saroyan, A.I. Dovgiy // Regenerativnaya biologiya i medicina, sbor.tr.FGBNU «Nauchno-issledovatel'skiy institut morfologii cheloveka»(FGBNU NIIMCh) – Moskva, 2021. – S. 60-61.
2. Kulyagina, Yu.I. Obosnovanie effektivnosti primeneniya autosyvorotki krovi pri yazvennyh porazheniyah rogovicy i sindrome sleznoy disfunkcii u melkih domashnih zhivotnyh: vypusknaya kvalifikacionnaya rabota / Yu.I. Kulyagina. – Moskva, Izdatel'stvo OOO «Kopirka», 2018. – 73 s.
3. Lukashina, U.E. Primenenie keratoplastiki v veterinarnoy oftal'mologii: vypusknaya kvalifikacionnaya rabota / U.E. Lukashina. – M., Izdatel'stvo OOO «Kopirka», 2018. – 125 s.
4. Pavlova, T.N. Keratoplastika v veterinarnoy oftal'mologii / T.N. Pavlova: dis. … kand. vet. nauk (data zaschity 27.06.2013). – M: Frantera, 2013. – 155 s.
5. Pavlova, T.N. Preimuschestva i nedostatki vseh dostupnyh materialov dlya keratoplastiki v veterinarnoy oftal'mologii na segodnyashniy den' / T.N. Pavlova // II Vserossiyskaya mezhvuzovskaya konferencii po veterinarnoy hirurgii: Tezisy dokladov. Moskva, 2011. – S. 125-129.
6. Pavlova, T.N. Sravnitel'nyy analiz ispol'zuemyh materialov dlya peresadki rogovicy v veterinarnoy oftal'mologii, preimuschestva i nedostatki / T.N. Pavlova // Aktual'nye voprosy veterinarnoy biologii. – 2012. – № 4. – S. 47-50.
7. Poltorackaya, O.I. Medicinskiy kley Sul'fakrilat / O.I. Poltorackaya // Ukrainskiy neyrohirurgicheskiy zhurnal. – 2014. – № 1. – S. 66-69.
8. Oleynik, V.V. Osobennosti terapii vospalitel'nyh zabolevaniy glaz u sobak i koshek s uchetom porodnyh faktorov / V.V. Oleynik, A.G. Shilkin // Veterinarnyy Peterburg. – 2013. – № 2. – S. 38-41.
9. Halimova, L.I. Ocenka effektivnosti akselerirovannogo i impul'snogo akselerirovannogo ul'trafioletovogo krosslinkinga rogovicy po harakteru demarkacionnoy linii stromy / L.I. Halimova // Oftal'mologiya. – 2019. – №16. – S. 127- 131.
10. Shilkin, A.G. Gnoynye yazvy rogovicy u sobak i koshek / A.G. Shilkin // Veterinariya. – 2004. – №5. – S. 53-55.
11. Shilkin, A.G. Sravnitel'naya effektivnost' razlichnyh glaznyh form ftorhinolonov v veterinarnoy oftal'mologii / A.G. Shilkin, E.P Kopenkin, V.V. Oleynik, S.V. Smirnova // Materialy XIV Moskovskogo mezhdunarodnogo veterinarnogo kongressa. – 2006. – S. 158-161.
12. Shilkin, A.G. Hirurgicheskoe lechenie yazv i travmaticheskih porazheniy rogovicy / A.G. Shilkin, D.A. Rotanov, D.V. Goncharov // Rossiyskiy veterinarnyy zhurnal. Melkie domashnie i dikie zhivotnye. – 2010. – № 1. – S. 17-19.
13. Shilkin, A.G. Otdalennye rezul'taty klinicheskogo primeneniya obogaschennoy trombocitami plazmy v veterinarnoy oftal'mologii / A.G. Shilkin, M.A. Voyteha, T.N. Pavlova, D.A. Rotanov, K.A. Novikova // Rossiyskiy veterinarnyy zhurnal. Melkie domashnie i dikie zhivotnye. – 2017. – № 5. – S. 9-10.
14. Famose, F. Evaluation of accelerated collagen cross-linking for the treatment of melting keratitis in eight dogs / F. Famose // Veterinary Ophthalmology. – 2014. – No. 5 (17). – R.358-367.
15. Famose, F. Evaluation of accelerated collagen cross-linking for the treatment of melting keratitis in ten cats / F. Famose // Veterinary Ophthalmology. – 2015. – No. 18(2). – pp. 95-104.
16. Hafezi, F. PACK-CXL: defining CXL for infectious keratitis / F. Hafezi, J.B. Randleman // J Refract Surg. – 2014. – No. 30(7). – pp. 438-439. DOI: 10/3928/1081597X-20140609-01.
17. Kaya, V. Intraoperative corneal thickness measurements during corneal collagen hypoosmolar riboflavin solution in thin corneas / V. Kaya, C.A. Utine, O.F. Yilmaz // Cornea. – 2012. – No. 31 (5). – pp. 486-490.
18. Kymionis, G.D. Corneal collagen crosslinking and herpetic keratitis / G.D. Kymionis, D.M. Portaliou // J Cataract Refract Surg. – 2013. – No. 39(8). – pp. 1281.
19. Maggs, D.J. Slatter’s fundamentals of veterinary ophthalmology, 4th edition. / D.J. Maggs, P.E. Miller, R. Ofri. – St. Louis: Saunders Elsevier, 2008. – 482 p.
20. Mazzotta, C. In vivo confocal microscope after contact lens-assisted thin keratoconic corneas / C. Mazzotta, S. Jacob // Refract surgery. – 2016. – No. 32 (5). – pp. 326-331.
21. Papaioannou, L. Corneal collagen Cross-linking for infectious keratitis: A systematic review and meta-analysis / L. Papaioannou, M. Miligkos, Papathanassiou M. // Cornea. – 2016. –No. 36(1). – pp. 62-71.
22. Pot, A. Corneal collagen cross-linking as treatment for infectious and noninfectious corneal melting in cats and dogs: results of a prospective, nonrandomized, controlled trial / A. Pot // Veterinary ophthalmology. – 2014. – No. 4 (17). – pp. 250-260.
23. Pot, A. Treatment of bullous keratopathy with corneal collagen cross-linking in two dogs / A. Pot, N.S. Gallhoeffer // Veterinary ophthalmology. – 2015. – No. 2 (18). – pp. 168-173.
24. Said, D. Collagen Cross-Linking with Photoactivated Riboflavin (PACK-CXL) for the Treatment of Advanced Infectious Keratitis with Corneal Melting / D. Said, M. Elalfy, Z. Gatzioufas // Ophthalmology. – 2014. – No. 121(7). – pp. 1377-1382.
25. Seiler, T. Corneal cross-linking-induced stromal demarcation line / T. Seiler, F. Hafezi // Cornea. – 2006. – No. 25. – pp. 1057-1059.
26. Spiess, M.B. Corneal collagen cross-linking (CXL) for the treatment of melting keratitis in cats and dogs: a pilot study / M.B. Spiess, A.S. Pot, M. Florin, F. Hafezi // Veterinary Ophthalmology. – 2014. – No.1 (17). – pp. 1-11.
27. Spoerl, E. Safety of UVA- riboflavin cross-linking on the cornea / E. Spoerl // Cornea. – 2007. – No. 26(4). – pp. 385-389.
