BIOPHYSICAL PRINCIPLES IN HUMAN GAMETES CRYOPRESERVATION: CRYOPROTECTANTS MODIFIED BY EGG YOLK

A A Grigoreva; S B Garmaeva; S A Yakovenko; E Y. Simonenko; Vsevolod A. Tverdislov; A A Dolganova; V P Apryshko

doi:doi:

UDC 577

BIOPHYSICAL PRINCIPLES IN HUMAN GAMETES CRYOPRESERVATION: CRYOPROTECTANTS MODIFIED BY EGG YOLK

Published в Russian Journal of Biological Physics and Chemisrty · Volume 1, Issue 1, 2016 · Pages 70–73 · Rubrics: General biophysics

Received: 20.06.2016 Accepted: 20.06.2016 Published: 25.06.2016 Language of publication: RUS

Authors

A A Grigoreva ¹ · S B Garmaeva ² · S A Yakovenko ³ · E Y. Simonenko ⁴ · Vsevolod A. Tverdislov ⁵ · A A Dolganova ⁶ · V P Apryshko ⁷

1 Lomonosov Moscow State University

2 Lomonosov Moscow State University

3 Lomonosov Moscow State University; IVF Clinic AltraVita

4 Lomonosov Moscow State University

5 Lomonosov Moscow State University (Department of Biophysics, Faculty of Physics, Head of the Department)
Moscow, Moscow, Russian Federation

6 IVF Clinic AltraVita

7 IVF Clinic AltraVita

ABSTRACT

Cryopreservation of human gametes is one of the most important methods of IVF allowing to save functional gametes prior to the treatment which might threaten male fertility. However, fertilizing capacity of spermatozoa after cryopreservation decreases by 30-70 % on average, explaining the relevance of search for new substances and protocols possessing improved cryoprotective properties. In this work we suggested modification of cryoprotectant by addition of specifically prepared egg yolk emulsion. Such modification showed positive influence on safety of fertile spermatozoa, - increasing the mean number of normal motile gametes after cryopreservation by 20 % and decreasing morphological defectiveness. The emulsion was tested for cytotoxicity and compared with commercially available cryoprotectant containing egg yolk.

KEYWORDS

gametes cryoprotectant spermatozoa IVF cryopreservation

Information Text References

Authors:

1. Lomonosov Moscow State University

2. Lomonosov Moscow State University

3. Lomonosov Moscow State University; IVF Clinic AltraVita

4. Lomonosov Moscow State University

5. Lomonosov Moscow State University (Department of Biophysics, Faculty of Physics, Head of the Department)

Moscow, Moscow, Russian Federation

6. IVF Clinic AltraVita

7. IVF Clinic AltraVita

Type:

Article

Pages:

from 70 to 73

Status:

Published

Subject area:

UDC 577

Language:

Russian

Keywords:

gametes, cryoprotectant, spermatozoa, IVF, cryopreservation

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References

1. Garde J.J., Olmo A., Soler A.J., Espeso G., Roldan E.R.S., Gomendio M. Effect of egg yolk, cryoprotectant, and various sugars on semen cryopreservation in endangered Cuvier’s gazelle (Gazella cuvieri). Animal Reproduction Science, 2008, no. 108, pp. 384-401.

2. Salmani H., Towhidi A., Zhandi M., Bahreini M, Sharafi M. In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology, 2014, no. 68, pp. 276-280.

3. Yildiz C., Bozkurt Y., Yavas I. An evaluation of soybean lecithin as an alternative to avian egg yolk in the cryopreservation of fish sperm. Cryobiology, 2013, no. 67, pp. 91-94. DOI: https://doi.org/10.1016/j.cryobiol.2013.05.008; EDN: https://elibrary.ru/YDKAIT

This work is licensed under Creative Commons Attribution 4.0 International

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This work is licensed under Creative Commons Attribution 4.0 International Creative Commons

BIOPHYSICAL PRINCIPLES IN HUMAN GAMETES CRYOPRESERVATION: CRYOPROTECTANTS MODIFIED BY EGG YOLK

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