It was shown that acridine orange (AO, ANS) introduction into the living yeast cells promotes the appearance of excitable msec-delayed light emmision (DLE) in them. The kinetics of delayed light emission AO, ANS in yeast cells has inductive character, is described by S-shaped curve, and its intensity depends on the dye concentration. At work was used photometric installation allowing to register msec-DLE. At our investigation work has been studied msec-DLE AO intensity in yeast cells after γ-irradiation of various doses (5 gr, 20 gr, 50 gr, 100 gr). It was determined that, the kinetic intensity indications of msec-DLE AO change depending on γ -irradiation doses. The investigation of γ-irradiation on msec-DLE AO in cells showed that, msec-DLE AO intensity in cells previously illuminated decreases compared with non-irradiated ones and changes the form of induction curve under the influence 5 gr. dose. It was identified that, after γ-irradiation influence on yeast cells at low doses, practically not causing any damage, msec-DLE AO increase. By increasing irradiation doses msec-DLE ANS intensity decreases. It was supposed that, msec-DLE AO and ANS can be used to investigate the influence of biogenic and not biogenic factors of medium in biological systems, and also for biomonitoring of pollution in environmental medium with the aim of early diagnostics.
yeast cells, AO, ANS, msec-DLE, γ-irradiation
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