In recent years, much research has been devoted to the development of effective systems for the delivery of essential polyunsaturated fatty acids ([omega] - 3, [omega] - 6) and various functional nutraceuticals (antioxidants, vitamins, polyphenols, etc.) to the human body through food systems. Some of these systems are soy phosphatidylcholine (PC) liposome-based nanocoplexes with included nutraceuticals, long-term consumption of which can affect the antioxidant status of various organs and tissues of living organisms. In the present work, the method of thermoinitiated chemiluminescence (TIC) was used to study the change in antioxidant activity (AOA) of brain lipids of six mice groups depending on the composition of liposome nanocoplexes introduced into their long-term (3 months) diet. The components of six types of liposomal nanocomplexes, except for PC, in different combinations were: clove essential oil (CEO), fish oil and sodium caseinate (Cas-Na). The results of the study showed that nanocomplexes containing liposomes from PC with the addition of fish oil, CEO and encapsulated milk protein (Cas-Na) proved to be the most effective in increasing mouse brain lipid AOA compared to control.
functional nutraceuticals, liposome, essential oil cloves, fish oil, sodium caseinate, antioxidant, chemiluminescence
1. Burlakova E.B., Alesenko A.V., Molochkina E.M., Pal'mina N.P., Hrapova N.G. Bioantioksidanty v luchevom porazhenii i zlokachestvennom roste. M.: Nauka, 1975, 375 s. @@[Burlakova E.B., Alesenko A.V., Molochkina E.M., Palmina N.P., Khrapova N.G. Bioantioxidants in radiation damage and malignant growth. M.: Nauka, 1975, 375 p. (In Russ.)]
2. Burlakova E.B., Pal'mina N.P. Antioksidanty v himioterapii opuholey. Voprosy onkologii, 1990, t. 36, № 10, s. 1155-1165. @@[Burlakova E.B, Palmina N.P. Antioxidants in tumor chemotherapy. Voprosi oncologii, 1990, vol. 36, no. 10. pp. 1155-1165. (In Russ.)]
3. Popov I., Lewin G., Matthes G., Meffert H., Scherf H.P., Hengst P., Conradi E., Heilmann H., Zimmermann I., Baehr R.V. Zur Bedeutung der antioxidativen Kapazität des Blutplasmas. Z. Klin. Med, 1988, vol. 43, no. 19, pp. 1663-1668.
4. Popov I., Popov M., Lewin G., in Free Radicals and Oxidative Stress: Chemistry, biochemistry and pathophysiological implications. Ed. by D. Galaris Medimond, Ioannina, 2003, pp. 219-223.
5. Burlakova E.B., Hrapova N.G. Perekisnoe okislenie lipidov membran i prirodnye antioksidanty. Uspehi himii, 1985, t. 54, № 9, s. 1540-1558. @@[Burlakova E.B., Khrapova N.G. Peroxidation of membrane lipids and natural antioxidants. Russian Chem. Reviews, 1985, vol. 54, no. 9, pp. 907-917. (In Russ.)]
6. Burlakova E.B., Krashakov S.A., Hrapova N.G. Rol' tokoferola v peroksidnom okislenii lipidov biomembran. Biologicheskie membrany, 1998, t. 15, № 2, s. 137-168. @@[Burlakova E.B., Krashakov S.A., Khrapova N.G. Role of tocopherol in lipid peroxide oxidation of biomembran. Biologicheskie membrane, 1998, vol. 15, no. 2, pp. 137-168. (In Russ.)]
7. Dhavamani S., Rao Y.P.C., Lokesh B.R. Total antioxidant activity of selected vegetable oils and their influence on total antioxidant values in vivo: A photochemiluminescence based analysis. Food Chemistry, 2014, vol. 164, pp. 551-555.
8. Popov I.N., Levin G. Antiokislitel'naya sistema organizma i metod termoiniciirovannoy hemilyuminescencii dlya kolichestvennoy harakteristiki ee sostoyaniya. Biofizika, 2013, t. 58, № 5, s. 848-856. @@[Popov I.N., Levin G. An antioxidant system of an organism and a thermoinitiated chemiluminescence method for quantifying its condition. Biofizika, 2013, vol. 58, no. 5, pp. 848-856. (In Russ.)]
9. Saini R.K., Keum Y.-S. Omega-3 and omega-6 polyunsaturated fatty acids: Dietary sources, metabolism, and significance. Life Sciences, 2018, vol. 203, pp. 255-267.
10. Mocking R.J.T., Assies J., Ruhé H.G., Schene A.N., Focus on fatty acids in the neurometabolic pathophysiology of psychiatric disorders. J. of Inherited Metabolic Disease, 2018, vol. 41, pp. 597-611.
11. Simonetto M., Infante M., Sacco R.L., Rundek T., Della-Morte D. A Novel Anti-Inflammatory Role of Omega-3 PUFAs in Prevention and Treatment of Atherosclerosis and Vascular Cognitive Impairment and Dementia. Nutrients, 2019, vol. 11, pp. 2279-2285.
12. Candela G.C., M.Bermejo L.L., Loria K.V. Changes in the plasma and erythrocytes lipid profile from overweight/obese anddyslipidemic patients after consumption of a naturally PUFA-enriched cheese. Nutric. Hospital, 2011, vol. 26, no. 2, pp. 323.
13. Semenova M.G., Antipova A.S., Pal'mina N.P., Misharina T.A., Martirosova E.I., Zelikina D.V., Krikunova N.I., Kasparov V.V., Binyukov V.I., Bogdanova N.G., Chebotarev S.A., Gureeva M.D. Kompleksy biopolimerov s essencial'nymi lipidami: vzaimosvyaz' struktury i funkcional'nyh svoystv. Himicheskaya fizika, 2019, t. 38, № 12, s. 38-43. @@[Semenova M.G., A.S. Antipova A.S., Palmina N.P., Misharina T.A., Martirosova E.I., Zelikina D.V., Krikunova N.B., Kasparov V.V., Binyukov V.I., Bogdanova N.G., Chebotarev S.A., Gureeva M.D. Complexes of biopolymers with essential lipids: relationship of structure and functional properties. Russian Chem. Physics, 2019, vol. 38, no. 12, pp. 38-43. (In Russ.)]
14. Semenova M.G., Antipova A.S., Misharina T.A., Alinkina E.S., Zelikina D.V. , Martirosova E.I., Palmina N.P., Binyukov V.I. , Maltseva E.L., Kasparov V.V. , Ozerova N.S., Shumilina E.A., Baeva K.A., Bogdanova N.G. Gums and Stabilisers for the Food Industry 18: Hydrocolloid Functionality for Affordable and Sustainable Global Food Solutions. Cambridge, Publ.: Royal Soc. of Chem., 2016, 182 p.
15. Misharina T.A., Fatkullina L.D., Alinkina E.S., Kozachenko A.I., Nagler L.G., Medvedeva I.B., Goloschapov A.N., Burlakova E.B. Vliyanie priema malyh doz efirnyh masel na antioksidantnyy status eritrocitov, pecheni i mozga myshey. Prikl biohimiya i mikrobiologiya, 2014, t. 50, № 1, s. 1-7. @@[Misharina T.A., Fatkullina L.D., Alinkina E.S., Kozachenko A.I., Nagler L.G., Medvedeva I.V., Goloshchapov A.N., Burlakova E.B. Effect of administration of small essential oils doses on the antioxidant status of erythrocytes, liver and brain of mice. Prikl. Biokhim. Mikrobiol., 2014, vol. 50, no.1, pp. 1-7. (In Russ.)]
16. Valentini K.J., Pickens C.A., Wiesinger J.A., Fenton J.I. The Effect of Fish Oil Supplementation on Brain DHA and EPA Content and Fatty Acid Profile in Mice. International Journal of Food Sciences and Nutrition, 2018, vol. 69, no. 6, 705-717. DOI: 10.1080/09637486.2017.1413640.
17. Balcerezyk A., Gajewska A., Macierzynska-Piotrowska E., Pawelezyk T., Bartosz G., Szemraj J. Enhanced Antioxidant Capacity and Anti-Ageing Biomarkers after Diet Micronutrient Supplementation. Molecules, 2014, vol. 19, pp. 14794-14799. DOI: 10.3390/molecules190914794.
18. Keyts M. Tehnika lipidologii. Vydelenie, analiz i identifikaciya lipidov. M.: Mir, 1975. @@[Keyts M. Lipid isolation, analysis and identification. M.: Mir, 1975. (In Russ.)]
19. Popov I., Lewin G. Oxidierbarkeit und ihre Quantifizierung. Medikobiologische Aspekte. LABO. 1997, vol. 11, pp. 8-10.
20. Popov I., Lewin G. Antioxidative homeostasis, its evaluation by means of chemiluminescent methods. In: Handbook of chemiluminescent methods in oxidative stress assessment. Transworld Research Network. Kerala, 2008, pp. 361-391.
21. Sazhina N.N., Popov I.N., Titov V.N. Sravnenie dvuh hemilyuminescentnyh modeley dlya ocenki antiokislitel'noy aktivnosti syvorotki krovi pacientov s patologiey pecheni. Klin. labor. Diagnostika, 2018, t. 63, № 1, s. 16-21. @@[Sazhina N.N., Popov I.N., Titov V.N. The comparison of two chemiluminescent models for assessing antioxidative activity of blood serum of patients with liver pathology. Klinicheskaya laboratornaya diagnostika, 2018, vol. 63, no. 1, pp. 16-21. (In Russ.)]
22. Burlakova E.B., Molochkina E.M., Pal'mina N.P., Slepuhina L.V., Izmenenie antiokislitel'noy aktivnosti lipidov pri starenii. Voprosy med. Himii, 1976, t. 22, № 4, s. 541-546. @@[Burlakova E.B., Molochkina E.M., Palmina N.P., Slepuchina L.V. Change in the antioxidant activity of lipids during aging. Vopr. Med. Chemistry, 1976, vol. 22, no. 4, pp. 542-546. (In Russ.)]
23. Pal'mina N.P., Obuhova L.K., Bunto T.V. Antiokislitel'naya aktivnost' lipidov v processe stareniya myshey i pri vozdeystvii geroprotektora antioksidanta. Izvestiya AN SSSR. Ser. Biol., 1979, t. 2, s. 290-293. @@[Palmina N.P., Obuchova L.K., Bunto T.V. Antioxidant activity of lipids during aging of mice and when exposed by heroprotective antioxidant. Izvestia AN SSSR, Ser. Biol., 1979, vol. 2, pp. 290-293. (In Russ.)]
24. Sazhina N.N., Antipova A.S., Semenova M.G., and Palmina N.P. Initiated Oxidation of Phosphatidylcholine Liposomes with Some Functional Nutraceuticals. Russian Journal of Bioorganic. Chemistry, 2019, vol. 45, no. 1, p. 34, DOI: 10.1134/S1068162019010138.
25. Rodrigo R., Prieto J.C., Castillo R. Cardioprotection against ischaemia/reperfusion by vitamins C and E plus n-3 fatty acids: molecular mechanisms and potential clinical applications. Clinical Science, 2013, vol. 124, pp. 1-15.
