In our work, we studied the biometric indicators and the content of photosynthetic pigments in radish seedlings ( Raphanus sativus var. Radicula ), the seeds of which were treated with solutions of chitosan of various concentrations in various organic acids (acetic, ascorbic, lactic) with the addition of complex fertilizer, and X-ray radiation (characteristic Mo radiation, accelerating voltage 20 kV, current 1 mA). In this case, the germination rate of unirradiated seeds was 50%, irradiated for 20 min - 80%, for 40 min - 20%. Seeds treated with 0.2% and 2% solutions of chitosan in 1% acetic and lactic acids did not germinate at all. Seeds treated with a 0.2% solution of chitosan in ascorbic acid and irradiated, sprouted, although their germination was lower - 10-20%. At the same time, the highest concentration of photosynthetic pigments was found in these sprouts. Seed treatment with mineral fertilizer followed by drying and irradiation led to the decrease in germination in the experimental irradiated variants: germination was 20% and 30% depending on the dose (20 and 40 min, respectively) when irradiated after treatment; 30 and 40% when irradiated before treatment. The germination rate of seeds inlaid with a 2% solution of chitosan in 2% ascorbic acid amounted to 50%, irradiated in various doses - 10%. Irradiation of seeds in most cases leads to a decrease in the content of the main photosynthetic pigments in seedlings. However, in the case of using mineral fertilizers and a solution of chitosan in ascorbic acid in combination with irradiation, on the contrary, it leads to an increase in the content of pigments in comparison with the control.
X-ray radiation, radiation technology, chitosan, seed treatment, Raphanus sativus
1. Kudryashov Yu.B. Radiacionnaya biofizika (ioniziruyuschie izlucheniya). M.: Fizmatlit, 2004, 448 s.
2. Zhuravskaya A.N., Voronov I.V., Poskachina E.R., Slepcov I.V. Vliyanie gamma-oblucheniya i liofilizata schiricy zaprokinutoy na fotosintez prorostkov pshenicy. Nauka i obrazovanie, 2015, № 3.
3. Morozova E.A. Ekologo-biologicheskaya ocenka deystviya hitozanovyh preparatov na sel'skohozyaystvennye kul'tury. Avtoreferat dissertacii na soiskanie uchenoy stepeni kandidata biologicheskih nauk. Barnaul, 2011.
4. Mahdavi B., Rahimi A. Seed priming with chitosan improves the germination and growth performance of ajowan (Carum copticum) under salt stress. Eurasian Journal of Biosciences, 2013, vol. 7. DOI: 10.5053/ejobios.2013.7.0.9.
5. Defang Zeng, Xinrong Luo, Renjie Tu Application of bioactive coatings based on chitosan for soybean seed protection. International Journal of Carbohydrate Chemistry, 2012, Article ID 104565. DOI: 10.1155/2012/104565.
6. Martínez González L., Reyes Guerrero Y., Falcón Rodríguez A., Núñez Vázquez M. Effect of seed treatment with chitosan on the growth of rice (Oryza sativa L.) seedlings cv. INCA LP-5 in saline medium. Cultivos Tropicales, 2015, vol. 36, no. 1, pp. 136-142.
7. Akinshina N.G., Rashidova D.K., Azizov A.A. Kapsulirovanie semyan preparatami hitozana i ego proizvodnyh vosstanavlivaet fotosintez u rasteniy hlopchatnika (Gossypium L., 1753) na fone vilta. Sel'skohozyaystvennaya biologiya, 2016, t. 51, № 5, s. 696-704. DOI: 10.15389/agrobiology.2016.5.696rus. [
8. Borisenko V.V., Zholobova I.S. Izuchenie vliyaniya obogaschennogo biogumata «Ekoss» na rabotu fotosinteticheskogo kompleksa rasteniy redisa. Nauchnyy zhurnal KubGAU, 2015, № 107 (03).
9. Muley A.B., Shingote P.R., Patil A.P., Dalvi S.G., Suprasanna P. Gamma radiation degradation of chitosan for application in growth promotion and induction of stress tolerance in potato (Solanum tuberosum L.). Carbohydrate Polymers, 2019, vol. 210, pp. 289-301. DOI: 10.1016/j.carbpol.2019.01.056.
10. Mar'ina-Chermnyh O.G., Evdokimova M. A. Effektivnost' novogo sposoba poseva i predposevnoy obrabotki semyan redisa. Vestnik Mariyskogo gosudarstvennogo universiteta. Seriya «Sel'skohozyaystvennye nauki. Ekonomicheskie nauki», 2015, № 4.
11. Loy N.N., Sanzharova N.I., Kuznecov A.A., Molin A.A., Vinokurov V.A. Effektivnost' predposevnoy obrabotki semyan sel'skohozyaystvennyh kul'tur produktami pererabotki, poluchennymi v rezul'tate radiacionnyh tehnologiy. Dostizheniya nauki i tehniki APK, 2015, t. 29, № 5, c. 29-32.
12. Kudryashov A.P. Fiziologiya rasteniy. Laboratornyy praktikum. Minsk: BGU, 2011, 76 s.
