The influence of 5-azacitidine (5A) DNA-demethylation agent on Amur grapes (Vitis amurensis Rupr) cellular culture with the help of karyological indicators is described in the article. The chromosomal variability increase and the kernel relation reduction as the result of 5A processing in various studied culture concentrations are determined. These processes testify the cells protein biosynthesis activation.
5-azacitidine, cytosine DNA methylation, Vitis amurensis, karyological indicators
1. Vanyushin B.F., Ashapkin V.V. DNA methylation in higher plants: Past, present and future // Biochim. Biophys. Acta. - 2011. - № 1809. - P. 360-368.
2. Law J., Jacobsen S.E. Establishing, maintaning and modifying DNA methylation patterns in plants and animals // Nat. - 2010. - Vol. 11. - P. 204-220.
3. Goll M.G., Bestor T.H. Eukaryotic cytosine methyltransferases // Annu. Rev. Biochem. - 2005. - № 74. -P. 481-514.
4. Zhu J.K. Active DNA demethylation mediated by DNA glycosylases // Annu Rev Genet. - 2009. - Vol. 43. -P. 143-166.
5. Meyer P. DNA methylation systems and targets in plants // FEBS Lett. - 2011. - Vol. 585. - P. 2008-2015.
6. Uvelichenie belkovosti zernovok pshenic pod vliyaniem 5-azacitidina - ingibitora metilirovaniya DNK / B.F. Vanyushin [i dr.] // Izvestiya AN SSSR. Ser. biol. - 1990. - № 1. - S. 75-83.
7. Morgun V.V. Eksperimental'nyy mutagenez i ego ispol'zovanie v selekcii kukuruzy - Kiev: Naukova dumka - 1983. - C. 278.
8. Grape genome browser. - URL: http://www.genoscope.cns.fr.
9. The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells / K.V. Kiselev [et al.] // Biotechnology J. - 2007. - Vol. 128. - P. 681-692.
10. Kiselev K.V., Dubrovina A.S., Bulgakov V.P. Phenylalanine ammonia-lyase and stilbene synthase gene expression in rolB transgenic cell cultures of Vitis amurensis // Appl. Microbiol. Biotechnol. - 2009. - Vol. 82. -P. 647-655.
11. Muratova E.N. Metodiki okrashivaniya yadryshek dlya kariologicheskogo analiza hvoynyh // Bot. zhurn. - 1995. - T. 80. - № 2. - S. 82-86.
12. Smirnov Yu.A. Uskorennyy metod issledovaniya somaticheskih hromosom plodovyh // Citologiya. -1968. - T. 10. - № 12. - S. 1601-1602.
13. Weber H., Ziechmann C., Graessmann A. In vitro DNA methylation inhibits gene expression in transgenic tobacco // EMBO J. - 1990. - Vol. 9. - P. 4409-4415.
14. A high quality draft consensus sequence of the genome of a heterozygous grapevine variety / R. Velasco [et al.] // PLoS One. - 2007. - Vol. 12. - P. 1326.
15. Geneticheskaya izmenchivost' kallusnyh liniy zhen'shenya Panax ginseng / M.M. Kozyrenko [i dr.] // Biotehnologiya. - 2001. - № 1. - S. 19-26.
16. Chisla hromosom zhen'shenya Panax ginseng C.A. Mey / L.S. Lauve [i dr.] // Bot. zhurn. - 2008. - T. 93, № 1. - S. 158-161.
17. Effect of 5-azacytidine and trichostatin A on somatic centromere association in wheat / M. Vorontsova [et al.] // Genome. - 2004. - Vol. 47. - P. 399-403.
18. Shahbazov V.G., Shestopalova N.G. Nekotorye osobennosti yadryshka i yadra v kletkah gibridnogo luka // Dokl. AN SSSR. - 1971. - T. 196, № 5. - S. 1207-1208.