The data on Holstein bulls (n = 24) were ana- lyzed by 12 microsatellite loci (according to Interna- tional ISAG Nomenclature) owned by JVC ‘Krasnoyarskagroplem’. 5 alleles of 67 had a fre- quency of more than 55 %: BM1 818266 (56 %), BM1824188 (60 %), ETH10219 (60 %),SPS115248 (77 %), TGLA126117 (70 %) in bulls. 7alleles were also identified to be found in 30-40 % of animals: BM2113125 (33 %), ETH3117 (42 %), ETH3129 (38 %), ETH225148 (33 %), ETH225150 (31%), INRA23210 (44 %), TGLA122143 (38 %). 2% ofthe bulls possessed the following alleles:BM2113137, ETH10213, ETH225146, SPS115260, TGLA53162, 178, 184, TGLA122159, 161, 171, TGLA22781.The number of alleles (Na) in the TGLA 126 locus was an average of 5.58 alleles per locus. Quantita- tively the degree of polymorphism was identified using two indicators -the level of heterozygosity (H) and the information of polymorphism index (PIC). According to the data of 12 microsatellite loci stud- ied in 24 manufacturing bulls, the heterozygosity values of the observed (Ho) and expected (He) var- ied between 0.417-0.875 and 0.397-0.852, re- spectively. In the study, the average PIC per locus was 0.608 and ranged from 0.369 (SPS 115) to0.812 (TGLA53). From 12 markers 10 were highly polymorphic; their PIC values were higher than 0.5 that was a favorable indicator of genetic variability. The greatest indicator of PIC was in TGLA53 locus0.812, the smallest was in the locus TGLA126 -0.369. The greatest value of degree of observed heterozygosity (Ho) in Holstein breed was revealed in loci of ETH225, TGLA53, TGLA227 - 0.875.
Holstein breed, microsatellites, loci, alleles
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