Cel': ocenit' primenimost' metoda fluorescentnogo vyyavleniya produkta mitohondrial'nogo vosstanovleniya rezazurina – rezarufina dlya ocenki zhiznesposobnosti kul'tury fibroblastov legkih MRC-5 eksponirovannyh pylevymi chasticami ugol'noporodnogo proishozhdeniya ot 10 mkm do 100 nm. Nakoplenie produkta reakcii proishodit lineyno, chto otrazhaet biohimicheskoy aktivnost'yu kletok. Dlya kazhdoy proby byli polucheny uravneniya vida y=ax+b, gde koefficient «a» proporcionalen aktivnosti i chislu zhivyh kletok. V to zhe vremya ryad uglerodnyh produktov mozhet iskazhat' rezul'taty.
rezazurin, rezarufin, krasitel' alamarBlue, ugol'no-porodnye chasticy, RM10, MRC-5
1. Lavogina D. et al. Revisiting the Resazurin-Based Sensing of Cellular Viability: Widening the Application Horizon // Biosensors. 2022. Vol. 12, № 4. P. 196.
2. Al-Nasiry S. et al. The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells // Human Reproduction. 2007. Vol. 22, № 5. P. 1304–1309.
3. Sharma N. et al. Evaluation of Anticancer activity of Silver Nanoparticles on the A549 Human Lung Carcinoma Cell Lines through Alamar Blue Assay // BIO-PROTOCOL. 2019. Vol. 9, № 1.
4. Kononenko V., Drobne D. In Vitro Cytotoxicity Evaluation of the Magnéli Phase Titanium Suboxides (TixO2x−1) on A549 Human Lung Cells // IJMS. 2019. Vol. 20, № 1. P. 196.
